Study to determine presence of resistance to second-line anti-tuberculosis drugs in Kenyan isolates.

Abstract

Tuberculosis (TB) is yet far from being controlled. Despite the fact that several reasons could be attributed to this, a significant contributing factor is the development of resistance to the currently available drugs due to the successful adaptation of the pathogen to these drugs. Second-line anti-TB drugs are being used for treatment of Multi-Drug Resistant TB (MDR-TB) patients.The purpose of this study was to investigate the presence of drug resistant strains of Mycobacterium tuberculosis (MTB) to second-line anti-TB drugs (SLDs) in first-line predetermined drug susceptibility isolates obtained from different studies carried out at the Centre for Respiratory Diseases Research (CRDR) between 2002 and 2007.A total of 216 MTB isolates including 78 first-line drug resistant isolates to individual and combined drugs and 138 first-line drugs susceptible isolates to all drugs were selected for this study. Of the 78 first-line resistant isolates, 25 isolates were MDR-TB strains. Resistant ratio and proportion methods were used to test. All the isolates were tested for susceptibility to four second-line drugs including cycloserine, gatifloxacin, ethionamide and kanamycin. UUssiinngg SS..PP..SS..SS.. ccoommppuutteerr ddaattaa aannaallyyssiiss pprrooggrraammmmee,, aannaallyyssiiss ooff ddaattaa wwaass ddoonnee uussiinngg cchhii--ssqquuaarree ttoo ccoommppaarree rreessiissttaannccee aanndd ssuusscceeppttiibbiilliittyy aammoonngg tthhee ddrruuggss,, aanndd ttoo ccoommppaarree rreessiissttaannccee aanndd ssuusscceeppttiibbiilliittyy bbeettwweeeenn tthhee ffiirrsstt--lliinnee ssuusscceeppttiibbllee aanndd rreessiissttaanntt iissoollaatteess ttoo sseeccoonndd--lliinnee aannttii--TTBB ddrruuggss..Of the 216 first-line isolates tested, 96.3% were sensitive, 2.2% were fully resistant and 1.5% had intermediate resistance. Of the 78 isolates tested, 94.9% were sensitive, 4.2% were fully resistant and 1% were intermediate resistant. Of the 138 isolates tested, 97.1% were sensitive, 1.1% were fully resistant and 1.8% were intermediate resistant. Drug resistance to second-lineanti-TB drugs was not statistically associated with previous first-line anti-TB drugs resistance, although the resistance level of second-line anti-TB drugs in the first-line resistant isolates was higher than in the first-line sensitive isolates.Resistance of MTB to second-line anti-TB drugs is present in Kenya. There was resistance to all the four second-line anti-TB drugs tested in this study, in both first-line resistant and sensitive isolates. There was no XDR-TB strain isolated.As resistant MTB increases in Kenya further studies are needed to evaluate second-line DST techniques and establish an appropriate one within the national policies. Since the samples used in this study may not be a representation of the current national status of drug resistance to second-line anti-TB drugs, a national surveillance is important to establish the prevalence of second-line anti-TB drugs resistance in the country.