STUDY ON THE EFFECT OF TEMPERATURE ON CALIBRATION PERFORMANCE OF SUCROSE IN AQUEOUS SOLUTION WITH RESPECT TO SUGAR CONCENTRATION

Abstract

The calibration performance on sugar concentration in aqueous is shown to shift when absorbance is collected over a range of temperature relative to calibration developed at a single temperature. The aqueous solutions of sugar were prepared using high purity, laboratory chemicals (HPLC) reagent sucrose in grade-3 de-ionized water. The variation of absorbance against sugar concentration monitored at varied temperature was found to have positive nonlinear correlation. The temperature of sugar solution correlated well with absorbance features with PLS, R2 found to be 0.810, 0.971, 0.973 and 0.980 for 5%, 15%, 25% and 40% concentrations respectively. This occurred due to the effect of temperature on mean kinetic energy and mean square displacement of sucrose particulates in the ambience.